Sorted by References

  Map 27


27 - AA
Proteins expressed during growth with aeration. Media contained LBK buffered with 100 mM HOMOPIPES, pH 4.4.



27 - AB
Proteins expressed during growth with aeration. Media contained LBK buffered with 100 mM MES-50 mM propionic acid, pH 6.



27 - AC
Proteins expressed during growth with aeration. Media contained LBK buffered with 100 mM MOPS, pH 7.0.



27 - AD
Proteins expressed during growth with aeration. Media contained LBK buffered with 100 mM AMPSO, pH 9.2.



27 - BA
Proteins expressed during anaerobic growth. Media contained LBK buffered with 100 mM HOMOPIPES, pH 4.4.



27 - BB
Proteins expressed during anaerobic growth. Media contained LBK buffered with 100 mM MES-50 mM propionic acid, pH 6.



27 - BC
Proteins expressed during anaerobic growth. Media contained LBK buffered with 100 mM MOPS, pH 7.0.



27 - BD
Proteins expressed during anaerobic growth. Media contained LBK buffered with 100 mM AMPSO, pH 9.2.





  Map 37


37 - A
2-DE analysis of E. coli proteins from Nutropin production fermentation.



37 - B
2-DE analysis of E. coli proteins from Nutropin blank fermentation.





  Map 38


38 - A
2-DE profile of E. coli proteins at the end of anti-CD18 blank run fermentation.



38 - B
2-DE analysis of E. coli proteins from Nutropin production fermentation.



38 - C
2-DE profile of E. coli proteins at the end of Nutropin blank run fermentation.



38 - D
2-DE profile of E. coli proteins at the end of VEGF blank run fermentation.



38 - E
2-DE profile of E. coli proteins at the end of IGF-1 blank run fermentation.





  Map 39


39 - A
Comparison of proteins identified by LC/LC MS and by two-dimensional gels.





  Map 64


64 - A
Autoradiogram of two-dimensional gels of protein extracts from E. coli MG1655 grown aerobically at 37C in M9 medium (untreated control sample).



64 - B
Autoradiogram of two-dimensional gels of protein extracts from E. coli MG1655 0.75h after CdCl2 addtion (cadmium-stressed cells).



64 - C
Autoradiogram of two-dimensional gels of protein extracts from E. coli MG1655 2.5h after CdCl2 addtion (cells growing after recovery from cadmium-induced growth arrest).





  Map 68


68 - A
The membrane proteins of the wild type strain.



68 - B
The membrane proteins of the E. coli transformant producing recombinant human cytochrome P450 2E1 and cytochrome P450 reductase



68 - CA
The membrane proteins of E. coli producing human cytochrome P450 2D6 were solubilized with 0.5% sodium cholate. Cholate was exchanged against 7 M urea, 2 M thiourea and 4% CHAPS.



68 - CB
The membrane proteins of E. coli producing human cytochrome P450 2D6 were solubilized with 0.5% sodium cholate. Cholate was exchanged against 7 M urea, 2 M thiourea and 1% Triton X-100.





  Map 70


70 - A
Periplasmic E.coli proteins (PECP)



70 - B
E.coli cell lysate (ECP)





  Map 97


97 - A
2D gel of soluble protein fraction of BL21(DE3) at the exponential growth phase (blank fermentation).



97 - B
2D gel of soluble protein fraction of BL21(DE3) (pEDob5), which overproduces leptin, before induction.



97 - C
2D gel of soluble protein fraction of BL21(DE3) (pEDob5), which overproduces leptin, 9h after induction.



97 - D
2D gel of insoluble protein fraction of BL21(DE3) (pEDob5), which overproduces leptin, 9h after induction.



97 - E
2D gel of soluble protein fraction of BL21(DE3) (pEDob5 and pACYC104CysK), which overproduces leptin and CysK, before induction.



97 - F
2D gel of soluble protein fraction of BL21(DE3) (pEDob5 and pACYC104CysK), which overproduces leptin, 2h after induction.





  Map 99


99 - A
Proteome expression profile of E. coli XL1-Blue grown in LB medium.



99 - B
Proteome expression profile of E. coli XL1-Blue grown in LB medium plus 20 g/L of glucose.



99 - C
Proteome expression profile of E. coli XL1-Blue (pJC4) grown in LB medium.



99 - D
Proteome expression profile of E. coli XL1-Blue (pJC4) grown in LB medium plus 20 g/L of glucose.





  Map 116


116 - A
Changes in the protein synthesis pattern of E. coli. TG1:p FGFB before/after induction of hFGF-2 production. Samples taken from the bioreactor before induction were pulse-labeled with 35S-methionine and subjected to 2D gel electrophoresis and exposed autoradiogram.



116 - B
Changes in the protein synthesis pattern of E. coli. TG1:p FGFB before/after induction of hFGF-2 production. Samples taken from the bioreactor 30 min after induction were pulse-labeled with 35S-methionine and subjected to 2D gel electrophoresis and and exposed autoradiogram.





  Map 134


134 - A
Influence on the cold shock response. The exposed autoradiogram of 2D gel electrophoresis of the parent strain, E. coli MG1655.



134 - B
Influence of overexpression of CS7.4 on the cold shock response. The exposed autoradiogram of 2D gel electrophoresis of the E. coli MG1655:pJ3, which is the overexpression vector of the CS7.4 protein.





  Map 138


138 - AA
Two-dimensional protein pattern of the soluble protein fraction of E. coli RB791 (pKK177glucC, pUBS520) overproducing the a-glucosidase of S. cerevisiae before induction with IPTG.



138 - AB
Two-dimensional protein pattern of the soluble protein fraction of E. coli RB791 (pKK177glucC, pUBS520) overproducing the a-glucosidase of S. cerevisiae 3h after induction with IPTG.



138 - B
Two-dimensional protein pattern of the insoluble protein fraction of the overproduced a-glucosidase 3h after induction with IPTG.





  Map 145


145 - AA
Abbreviation: N4 The proteins of E. coli K12 showing significant difference in intracellular protein level (i.e., spot intensity) during the whole period of low-temperature growth in fed-batch culture. non-temperature-downshifted culture at 4 h after temperature downshift.



145 - AB
Abbreviation: N9 The proteins of E. coli K12 showing significant difference in intracellular protein level (i.e., spot intensity) during the whole period of low-temperature growth in fed-batch culture. non-temperature-downshifted culture at 9 h after temperature downshift.



145 - AC
Abbreviation: N15 The proteins of E. coli K12 showing significant difference in intracellular protein level (i.e., spot intensity) during the whole period of low-temperature growth in fed-batch culture. non-temperature-downshifted culture at 15 h after temperature downshift.



145 - BA
Abbreviation: C4 The proteins of E. coli K12 showing significant difference in intracellular protein level (i.e., spot intensity) during the whole period of low-temperature growth in fed-batch culture. temperature-downshifted culture at 4 h after temperature downshift.



145 - BB
Abbreviation: C9 The proteins of E. coli K12 showing significant difference in intracellular protein level (i.e., spot intensity) during the whole period of low-temperature growth in fed-batch culture. temperature-downshifted culture at 9 h after temperature downshift.



145 - BC
Abbreviation: C15 The proteins of E. coli K12 showing significant difference in intracellular protein level (i.e., spot intensity) during the whole period of low-temperature growth in fed-batch culture. temperature-downshifted culture at 15 h after temperature downshift.





  Map 154


154 - A
Identified E. coli membrane protein spots by peptide mass fingerprinting.





  Map 155


155 - A
Proteins induced in E. coli W3110 by 20 mM benzoate at pH 6.5. 2-D gels of extracts of E. coli W3110 grown at 37C in glucose rich medium without benzoate at pH6.5.



155 - B
Proteins induced in E. coli W3110 by 20 mM benzoate at pH 6.5. 2-D gels of extracts of E. coli W3110 grown at 37C in glucose rich medium in the presence of benzoate at pH6.5.



155 - C
Proteins induced in E. coli W3110 by 20 mM benzoate at pH 6.5. 2-D gels of extracts of E. coli W3110 grown at 37C in glucose rich medium without benzoate at pH8.0.



155 - D
Proteins induced in E. coli W3110 by 20 mM benzoate at pH 6.5. 2-D gels of extracts of E. coli W3110 grown at 37C in glucose rich medium in the presence of benzoate at pH8.0.





  Map 158


158 - A
Two-dimensional gels of FB8 wild-type strain.



158 - B
Two-dimensional gels of hns mutant, BE1410 hns strain.



158 - C
Two-dimensional gels of lrp mutant, BE1613 lrp strain.



158 - D
Two-dimensional gels of pta mutant, BE1612 pta strain.





  Map 160


160 - A
Silver-stained two-dimensional gel patterns of E. coli W3110, the parent strain



160 - B
Silver-stained two-dimensional gel patterns of E. coli TF5015, an L-threonine-overproducing mutant strain





  Map 231


231 - A
52 identified protein in the following conditions: carbon source as glucose under aerobic conditions.



231 - B
52 identified protein in the following conditions: carbon source as glucose under microaerobic conditions.



231 - C
52 identified protein in the following conditions: carbon source as acetate under aerobic conditions.



231 - D
52 identified protein in the following conditions: carbon source as gluconate under aerobic conditions.



231 - E
52 identified protein in the following conditions: carbon source as glycerol under aerobic conditions.





  Map 239


239 - A
Two-dimensional gel electrophoresis mapping of E. coli, which was grown with 500 uM sulfate as the sole sulfur source.



239 - B
Two-dimensional gel electrophoresis mapping of E. coli, which was grown with 500 uM ethanesulfonate as the sole sulfur source.





  Map 274


274 - A
Proteins differentially expressed at each growth pH, relative to expression at pH 7.0. All cultures of E. coli W3110 were grown at 37C to an OD600 of 0.2 in LBK with 100 mM buffer, which pH is 4.9. Spots that are pink indicate proteins expressed to a higher level at the comparative pH than at pH 7.0, whereas spots that are green indicate proteins repressed.



274 - B
Proteins differentially expressed at each growth pH, relative to expression at pH 7.0. All cultures of E. coli W3110 were grown at 37C to an OD600 of 0.2 in LBK with 100 mM buffer, which pH is 6.0. Spots that are pink indicate proteins expressed to a higher level at the comparative pH than at pH 7.0, whereas spots that are green indicate proteins repressed.



274 - C
Proteins differentially expressed at each growth pH, relative to expression at pH 7.0. All cultures of E. coli W3110 were grown at 37C to an OD600 of 0.2 in LBK with 100 mM buffer, which pH is 8.0. Spots that are pink indicate proteins expressed to a higher level at the comparative pH than at pH 7.0, whereas spots that are green indicate proteins repressed.



274 - D
Proteins differentially expressed at each growth pH, relative to expression at pH 7.0. All cultures of E. coli W3110 were grown at 37C to an OD600 of 0.2 in LBK with 100 mM buffer, which pH is 9.1. Spots that are pink indicate proteins expressed to a higher level at the comparative pH than at pH 7.0, whereas spots that are green indicate proteins repressed.





  Map 311


311 - A
Proteins expressed in a culture of E. coli K12 during growth under glucose-excess batch.



311 - B
Proteins expressed in a culture of E. coli K12 during growth under glucose-limited continuous.





  Map 323


323 - A
pH-dependent protein profiles after anaerobic growth. In the layered view shown two composite images, one representing growth at pH 8.5 (pink) and one representing growth at pH 5.5 (green), are superimposed. Cultures grown in LBK buffered with 100 mM MES (pH 5.5) or 100 mM TAPS (pH 8.5).



323 - B
pH-dependent protein profiles after anaerobic growth. In the layered view shown two composite images, one representing growth at pH 8.5 (pink) and one representing growth at pH 5.5 (green), are superimposed. Cultures grown in LBK buffered with a mixture of 50 mM MES and 50 mM1TAPS for both pH 5.5 and pH 8.5.





  Map 325


325 - A
Two-dimensional protein expression map. Protens that identified by MALDI-TOF MS are labeled with red, and those identified by E. coli SWISS-2DPAGE are labeled with blue. E. coli W3110 0.88 g DCW/L.



325 - B
Two-dimensional protein expression map. Protens that identified by MALDI-TOF MS are labeled with red, and those identified by E. coli SWISS-2DPAGE are labeled with blue. E. coli W3110 3.5 g DCW/L.



325 - C
Two-dimensional protein expression map. Protens that identified by MALDI-TOF MS are labeled with red, and those identified by E. coli SWISS-2DPAGE are labeled with blue. E. coli W3110 12.7 g DCW/L.



325 - D
Two-dimensional protein expression map. Protens that identified by MALDI-TOF MS are labeled with red, and those identified by E. coli SWISS-2DPAGE are labeled with blue. E. coli W3110 40 g DCW/L.



325 - E
Two-dimensional protein expression map. Protens that identified by MALDI-TOF MS are labeled with red, and those identified by E. coli SWISS-2DPAGE are labeled with blue. E. coli W3110 62 g DCW/L.



325 - F
Two-dimensional protein expression map. Protens that identified by MALDI-TOF MS are labeled with red, and those identified by E. coli SWISS-2DPAGE are labeled with blue. E. coli W3110 72 g DCW/L.



325 - G
Two-dimensional protein expression map. Protens that identified by MALDI-TOF MS are labeled with red, and those identified by E. coli SWISS-2DPAGE are labeled with blue. E. coli W3110 74 g DCW/L.