Entry Information
Protein Name EntC
Accession No P0AEJ2
Protein description Isochorismate synthase entC
pI/Mw (Da)
Theoretical Experimental
5.49/42931.65 5.54/36396
Protein function and expression It is specific to the enterobactin biosynthetic pathway in the isochorismate synthase family.
Reference(s) Champion, K. M., J. C. Nishihara, J. C. Joly, and D. Arnott. 2001. Similarity of the Escherichia coli proteome upon completion of different biopharmaceutical fermentation processes. Proteomics 1:1133-1148. (NCBI)

2D gels information
Map ID38
Reference(s)Champion, K. M., J. C. Nishihara, J. C. Joly, and D. Arnott. 2001. Similarity of the Escherichia coli proteome upon completion of different biopharmaceutical fermentation processes. Proteomics 1:1133-1148. (NCBI)
Protein function and expression(s)Map: E
pI/Mw: 5.54/36396

E
2-DE profile of E. coli proteins at the end of IGF-1 blank run fermentation.
Swiss-Prot Database Reference

Entry information
Entry Name ENTC_ECOLI
Primary accession number P0AEJ2
Secondary accession numbers P10377 P77099 Q2MBK8 
Integrated into Swiss-Prot on 2005-12-20
Sequence was last modified on 2005-12-20 (Sequence version 1)
Annotations were last modified on 2006-09-05 (Entry version 9)
Name and origin of the protein
Protein name Isochorismate synthase entC
Synonyms EC 5.4.4.2 Isochorismate synthase entC
Isochorismate mutase
Gene name Name : entC
OrderedLocusName : b0593
From Escherichia coli [TaxID:562] [HAMAP proteome]
Taxonomy Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia;
References
[1] NUCLEOTIDE SEQUENCE [GENOMIC DNA].
strain = K12
DOI=10.1016/0378-1097(90)90120-F;PubMed=2110093[NCBI, ExPASy, EBI, Israel, Japan]
Elkins M.F. , Earhart C.F. ,
"Opacity factor from group A streptococci is an apoproteinase."
FEMS Microbiol. Lett.56:35-40(1988) 
[2] NUCLEOTIDE SEQUENCE [GENOMIC DNA].
strain = K12
PubMed=2536681[NCBI, ExPASy, EBI, Israel, Japan]
Ozenberger B.A. , Brickman T.J. , McIntosh M.A. ,
"Nucleotide sequence of Escherichia coli isochorismate synthetase gene entC and evolutionary relationship of isochorismate synthetase and other chorismate-utilizing enzymes."
J. Bacteriol.171:775-783(1989) 
[3] NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
strain = K12 / MG1655 / ATCC 47076

Chung E. , Allen E. , Araujo R. , Aparicio A.M. , Davis K. , Duncan M. , Federspiel N. , Hyman R. , Kalman S. , Komp C. , Kurdi O. , Lew H. , Lin D. , Namath A. , Oefner P. , Roberts D. , Schramm S. , Davis R.W. ,
"Sequence of minutes 4-25 of Escherichia coli."
Submitted (1997-01) to the EMBL/GenBank/DDBJ databases 
[4] NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
strain = K12 / MG1655 / ATCC 47076
DOI=10.1126/science.277.5331.1453;PubMed=9278503[NCBI, ExPASy, EBI, Israel, Japan]
Blattner F.R. , Plunkett G. III , Bloch C.A. , Perna N.T. , Burland V. , Riley M. , Collado-Vides J. , Glasner J.D. , Rode C.K. , Mayhew G.F. , Gregor J. , Davis N.W. , Kirkpatrick H.A. , Goeden M.A. , Rose D.J. , Mau B. , Shao Y. ,
"The complete genome sequence of Escherichia coli K-12."
Science277:1453-1474(1997) 
[5] NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
strain = K12 / W3110 / ATCC 27325 / DSM 5911
DOI=10.1038/msb4100049;
Hayashi K. , Morooka N. , Yamamoto Y. , Fujita K. , Isono K. , Choi S. , Ohtsubo E. , Baba T. , Wanner B.L. , Mori H. , Horiuchi T. ,
"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Mol. Syst. Biol.2:E1-E5(2006) 
[6] NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-33.PubMed=2139473[NCBI, ExPASy, EBI, Israel, Japan]
Brickman T.J. , Ozenberger B.A. , McIntosh M.A. ,
"Regulation of divergent transcription from the iron-responsive fepB-entC promoter-operator regions in Escherichia coli."
J. Mol. Biol.212:669-682(1990) 
[7] PARTIAL PROTEIN SEQUENCE, AND CHARACTERIZATION.DOI=10.1021/bi00458a012;PubMed=2139795[NCBI, ExPASy, EBI, Israel, Japan]
Liu J. , Quinn N. , Berchtold G.A. , Walsh C.T. ,
"Overexpression, purification, and characterization of isochorismate synthase (EntC), the first enzyme involved in the biosynthesis of enterobactin from chorismate."
Biochemistry29:1417-1425(1990) 
Comments
  • CATALYTIC ACTIVITY : Chorismate = isochorismate
  • PATHWAY : Siderophore biosynthesis; enterobactin biosynthesis
  • SUBUNIT : Monomer
  • INDUCTION : By iron starvation
  • SIMILARITY : Belongs to the isochorismate synthase family
  • Copyright
    Copyrighted by the UniProt Consortium, see http://www.uniprot.org/. Distributed under the Creative Commons Attribution-NoDerivs License.
    Cross-reference
    Sequence databases
    EMBL M36700;AAA18491.1;-;Genomic_DNA [EMBL/GenBank/DDBJ][CoDingSequence].
    M24142;AAA16100.1;-;Unassigned_DNA [EMBL/GenBank/DDBJ][CoDingSequence].
    U82598;AAB40793.1;ALT_INIT;Genomic_DNA [EMBL/GenBank/DDBJ][CoDingSequence].
    U00096;AAC73694.1;-;Genomic_DNA [EMBL/GenBank/DDBJ][CoDingSequence].
    AP009048;BAE76348.1;-;Genomic_DNA [EMBL/GenBank/DDBJ][CoDingSequence].
    X53274;CAA37371.1;-;Genomic_DNA [EMBL/GenBank/DDBJ][CoDingSequence].
    PIR JT0497;SYECIK.
    3D structure databases
    ModBase P0AEJ2
    Protein-protein interaction databases
    DIP P0AEJ2
    Enzyme and pathway databases
    BioCyc EcoCyc:ENTC-MONOMER;-.
    2D gel databases
    SWISS-2DPAGE Get region on 2D PAGE
    Organism-specific gene databases
    EchoBASE EB0257;-.
    EcoGene EG10261;entC.
    HOGENOM [Family / Alignment / Tree]
    Family and domain databases
    InterPro IPR004561;Isochor_synth.
    IPR005801;TRPE_1_chor_bd.
    Graphical view of domain structure
    Pfam PF00425;Chorismate_bind;1.
    Pfam graphical view of domain structure
    PIRSF PIRSF001502;Isochrsmt_synth;1.
    ProDom PD000779;Anth_synth_chor;1.
    [Domain structure / List of seq. sharing at least 1 domain]
    TIGRFAMs TIGR00543;isochor_syn;1.
    BLOCKS P0AEJ2
    Genome annotation databases
    GenomeReviews U00096_GR;b0593.
    Other
    ProtoNet P0AEJ2
    UniRef View cluster of proteins with at least 50% / 90% / 100% identity
    Keyword
    Complete proteome; Direct protein sequencing; Enterobactin biosynthesis; Ion transport; Iron; Iron transport; Isomerase; Transport;
    Features
    Feature table viewer Feature aligner
    Key  From   To Length  Description  FTId
    CHAIN    1   391    391  Isochorismate synthase entC  PRO_0000154144
    CONFLICT  305   306    SG -> TA: (in Ref. 1)  
    Sequence information
    Length: 391AA [This is the length of the unprocessed precursor] Molecular weight: 42932Da [This is the MW of the unprocessed precursor] CRC64: 62882569DFC41AC4[This is a checksum on the sequence]
            10         20         30         40         50         60
    MDTSLAEEVQ QTMATLAPNR FFFMSPYRSF TTSGCFARFD EPAVNGDSPD SPFQQKLAAL
            70         80         90        100        110        120
    FADAKAQGIK NPVMVGAIPF DPRQPSSLYI PESWQSFSRQ EKQASARRFT RSQSLNVVER
           130        140        150        160        170        180
    QAIPEQTTFE QMVARAAALT ATPQVDKVVL SRLIDITTDA AIDSGVLLER LIAQNPVSYN
           190        200        210        220        230        240
    FHVPLADGGV LLGASPELLL RKDGERFSSI PLAGSARRQP DEVLDREAGN RLLASEKDRH
           250        260        270        280        290        300
    EHELVTQAMK EVLRERSSEL HVPSSPQLIT TPTLWHLATP FEGKANSQEN ALTLACLLHP
           310        320        330        340        350        360
    TPALSGFPHQ AATQVIAELE PFDRELFGGI VGWCDSEGNG EWVVTIRCAK LRENQVRLFA
           370        380        390 
    GAGIVPASSP LGEWRETGVK LSTMLNVFGL H
    P0AEJ2 in FASTA format