Entry Information
Protein Name GalF
Accession No P0AAB6
Protein description UTP-glucose-1-phosphate uridylyltransferase
pI/Mw (Da)
Theoretical Experimental
5.73/32829.23 -
Protein function and expression Involved in lipopolysaccharide biosynthesis.
Reference(s) Lopez-Campistrous, A., P. Semchuk, L. Burke, T. Palmer-Stone, S. J. Brokx, G. Broderick, D. Bottorff, S. Bolch, J. H. Weiner, and M. J. Ellison. 2005. Localization, annotation & comparison of the Escherichia coli K-12 proteome under two states of growth. Mol. Cell. Proteomics 4:1205-1209. (NCBI)

Swiss-Prot Database Reference

Entry information
Entry Name GALF_ECOLI
Primary accession number P0AAB6
Secondary accession numbers P71245 P78083 P97192 
Integrated into Swiss-Prot on 2005-09-13
Sequence was last modified on 2005-09-13 (Sequence version 1)
Annotations were last modified on 2006-05-30 (Entry version 8)
Name and origin of the protein
Protein name UTP--glucose-1-phosphate uridylyltransferase
Synonyms EC 2.7.7.9 UTP--glucose-1-phosphate uridylyltransferase
UDP-glucose pyrophosphorylase
UDPGP
Alpha-D-glucosyl-1-phosphate uridylyltransferase
Uridine diphosphoglucose pyrophosphorylase
Gene name Name : galF
OrderedLocusName : b2042
SynonymName : wcaN
From Escherichia coli [TaxID:562] [HAMAP proteome]
Taxonomy Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia;
References
[1] NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
strain = K12 / W3110 / ATCC 27325 / DSM 5911
DOI=10.1093/dnares/3.6.379;PubMed=9097040[NCBI, ExPASy, EBI, Israel, Japan]
Itoh T. , Aiba H. , Baba T. , Fujita K. , Hayashi K. , Inada T. , Isono K. , Kasai H. , Kimura S. , Kitakawa M. , Kitagawa M. , Makino K. , Miki T. , Mizobuchi K. , Mori H. , Mori T. , Motomura K. , Nakade S. , Nakamura Y. , Nashimoto H. , Nishio Y. , Oshima T. , Saito N. , Sampei G. , Seki Y. , Sivasundaram S. , Tagami H. , Takeda J. , Takemoto K. , Wada C. , Yamamoto Y. , Horiuchi T. ,
"A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map."
DNA Res.3:379-392(1996) 
[2] NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
strain = K12 / MG1655 / ATCC 47076
DOI=10.1126/science.277.5331.1453;PubMed=9278503[NCBI, ExPASy, EBI, Israel, Japan]
Blattner F.R. , Plunkett G. III , Bloch C.A. , Perna N.T. , Burland V. , Riley M. , Collado-Vides J. , Glasner J.D. , Rode C.K. , Mayhew G.F. , Gregor J. , Davis N.W. , Kirkpatrick H.A. , Goeden M.A. , Rose D.J. , Mau B. , Shao Y. ,
"The complete genome sequence of Escherichia coli K-12."
Science277:1453-1474(1997) 
[3] CHARACTERIZATION.PubMed=8971705[NCBI, ExPASy, EBI, Israel, Japan]
Marolda C.L. , Valvano M.A. ,
"The GalF protein of Escherichia coli is not a UDP-glucose pyrophosphorylase but interacts with the GalU protein possibly to regulate cellular levels of UDP-glucose."
Mol. Microbiol.22:827-840(1996) 
Comments
  • CATALYTIC ACTIVITY : UTP + alpha-D-glucose 1-phosphate = diphosphate + UDP-glucose
  • PATHWAY : Lipopolysaccharide biosynthesis
  • SIMILARITY : Belongs to the prokaryotic UDPGP family
  • Copyright
    Copyrighted by the UniProt Consortium, see http://www.uniprot.org/. Distributed under the Creative Commons Attribution-NoDerivs License.
    Cross-reference
    Sequence databases
    EMBL U38473;AAC77853.1;-;Genomic_DNA [EMBL/GenBank/DDBJ][CoDingSequence].
    AP009048;BAA15896.1;-;Genomic_DNA [EMBL/GenBank/DDBJ][CoDingSequence].
    U00096;AAC75103.1;-;Genomic_DNA [EMBL/GenBank/DDBJ][CoDingSequence].
    PIR A64970;A64970.
    3D structure databases
    ModBase P0AAB6
    Protein-protein interaction databases
    DIP P0AAB6
    Enzyme and pathway databases
    BioCyc EcoCyc:G7093-MONOMER;-.
    2D gel databases
    SWISS-2DPAGE Get region on 2D PAGE
    Organism-specific gene databases
    EchoBASE EB3348;-.
    EcoGene EG13578;galF.
    HOGENOM [Family / Alignment / Tree]
    Family and domain databases
    InterPro IPR005774;GalF.
    IPR005835;NTP_transferase.
    Graphical view of domain structure
    Pfam PF00483;NTP_transferase;1.
    Pfam graphical view of domain structure
    TIGRFAMs TIGR01105;galF;1.
    BLOCKS P0AAB6
    Genome annotation databases
    GenomeReviews U00096_GR;b2042.
    Other
    ProtoNet P0AAB6
    UniRef View cluster of proteins with at least 50% / 90% / 100% identity
    Keyword
    Complete proteome; Kinase; Lipopolysaccharide biosynthesis; Nucleotidyltransferase; Transferase;
    Features
    Feature table viewer Feature aligner
    Key  From   To Length  Description  FTId
    CHAIN    1   297    297  UTP--glucose-1-phosphate uridylyltransferase  PRO_0000201349
    Sequence information
    Length: 297AA [This is the length of the unprocessed precursor] Molecular weight: 32829Da [This is the MW of the unprocessed precursor] CRC64: 6F85D6BB12C0C5A8[This is a checksum on the sequence]
            10         20         30         40         50         60
    MTNLKAVIPV AGLGMHMLPA TKAIPKEMLP IVDKPMIQYI VDEIVAAGIK EILLVTHASK
            70         80         90        100        110        120
    NAVENHFDTS YELESLLEQR VKRQLLAEVQ SICPPGVTIM NVRQGEPLGL GHSILCARPA
           130        140        150        160        170        180
    IGDNPFVVVL PDVVIDDASA DPLRYNLAAM IARFNETGRS QVLAKRMPGD LSEYSVIQTK
           190        200        210        220        230        240
    EPLDREGKVS RIVEFIEKPD QPQTLDSDIM AVGRYVLSAD IWPELERTQP GAWGRIQLTD
           250        260        270        280        290 
    AIAELAKKQS VDAMLMTGDS YDCGKKMGYM QAFVKYGLRN LKEGAKFRKG IEKLLSE
    P0AAB6 in FASTA format