Entry Information
Protein Name GcvT
Accession No P27248
Protein description Aminomethyltransferase; Glycine cleavage system T protein
pI/Mw (Da)
Theoretical Experimental
5.36/40015.52 -
Protein function and expression The glycine cleavage system catalyzes the degradation of glycine.
Reference(s) Lopez-Campistrous, A., P. Semchuk, L. Burke, T. Palmer-Stone, S. J. Brokx, G. Broderick, D. Bottorff, S. Bolch, J. H. Weiner, and M. J. Ellison. 2005. Localization, annotation & comparison of the Escherichia coli K-12 proteome under two states of growth. Mol. Cell. Proteomics 4:1205-1209. (NCBI)

2D gels information
Map ID68
Reference(s)Fountoulakis, M., and R. Gasser. 2003. Proteomic analysis of the cell envelope fraction of Escherichia coli. Amino Acids 24:19-41. (NCBI)
Protein function and expression(s)Map: A
Spot: 4


A
The membrane proteins of the wild type strain.
Swiss-Prot Database Reference

Entry information
Entry Name GCST_ECOLI
Primary accession number P27248
Secondary accession numbers Q2M9T6 
Integrated into Swiss-Prot on 1992-08-01
Sequence was last modified on 1997-11-01 (Sequence version 2)
Annotations were last modified on 2006-07-25 (Entry version 52)
Name and origin of the protein
Protein name Aminomethyltransferase
Synonyms EC 2.1.2.10 Aminomethyltransferase
Glycine cleavage system T protein
Gene name Name : gcvT
OrderedLocusName : b2905
From Escherichia coli [TaxID:562] [HAMAP proteome]
Taxonomy Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia;
References
[1] NUCLEOTIDE SEQUENCE [GENOMIC DNA].
strain = K12
PubMed=8219277[NCBI, ExPASy, EBI, Israel, Japan]
Stauffer L.T. , Ghrist A. , Stauffer G.V. ,
"The Escherichia coli gcvT gene encoding the T-protein of the glycine cleavage enzyme system."
DNA Seq.3:339-346(1993) 
[2] NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 1-20.
strain = K12 / W3110 / ATCC 27325 / DSM 5911
PubMed=8375392[NCBI, ExPASy, EBI, Israel, Japan]
Okamura-Ikeda K. , Ohmura Y. , Fujiwara K. , Motokawa Y. ,
"Cloning and nucleotide sequence of the gcv operon encoding the Escherichia coli glycine-cleavage system."
Eur. J. Biochem.216:539-548(1993) 
[3] NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
strain = K12 / MG1655 / ATCC 47076
DOI=10.1126/science.277.5331.1453;PubMed=9278503[NCBI, ExPASy, EBI, Israel, Japan]
Blattner F.R. , Plunkett G. III , Bloch C.A. , Perna N.T. , Burland V. , Riley M. , Collado-Vides J. , Glasner J.D. , Rode C.K. , Mayhew G.F. , Gregor J. , Davis N.W. , Kirkpatrick H.A. , Goeden M.A. , Rose D.J. , Mau B. , Shao Y. ,
"The complete genome sequence of Escherichia coli K-12."
Science277:1453-1474(1997) 
[4] NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
strain = K12 / W3110 / ATCC 27325 / DSM 5911
DOI=10.1038/msb4100049;
Hayashi K. , Morooka N. , Yamamoto Y. , Fujita K. , Isono K. , Choi S. , Ohtsubo E. , Baba T. , Wanner B.L. , Mori H. , Horiuchi T. ,
"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Mol. Syst. Biol.2:E1-E5(2006) 
[5] PROTEIN SEQUENCE OF 1-12.
strain = K12 / EMG2
PubMed=9298646[NCBI, ExPASy, EBI, Israel, Japan]
Link A.J. , Robison K. , Church G.M. ,
"Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12."
Electrophoresis18:1259-1313(1997) 
Comments
  • FUNCTION : The glycine cleavage system catalyzes the degradation of glycine
  • CATALYTIC ACTIVITY : [Protein]-8-S-aminomethyldihydrolipoyllysine + tetrahydrofolate = [protein]-dihydrolipoyllysine + 5,10-methylenetetrahydrofolate + NH(3)
  • SUBUNIT : The glycine cleavage system is composed of four proteins: P, T, L and H
  • SIMILARITY : Belongs to the gcvT family
  • Copyright
    Copyrighted by the UniProt Consortium, see http://www.uniprot.org/. Distributed under the Creative Commons Attribution-NoDerivs License.
    Cross-reference
    Sequence databases
    EMBL M97263;AAC36843.1;-;Unassigned_DNA [EMBL/GenBank/DDBJ][CoDingSequence].
    X73958;CAA52144.1;-;Genomic_DNA [EMBL/GenBank/DDBJ][CoDingSequence].
    U28377;AAA69073.1;-;Genomic_DNA [EMBL/GenBank/DDBJ][CoDingSequence].
    U00096;AAC75943.1;-;Genomic_DNA [EMBL/GenBank/DDBJ][CoDingSequence].
    AP009048;BAE76970.1;-;Genomic_DNA [EMBL/GenBank/DDBJ][CoDingSequence].
    PIR A56689;A56689.
    3D structure databases
    PDB 1VLO;X-ray;A=1-363[ExPASy/RCSB/EBI].
    Detailed list of linked structures
    ModBase P27248
    Protein-protein interaction databases
    DIP P27248
    Enzyme and pathway databases
    BioCyc EcoCyc:GCVT-MONOMER;-.
    2D gel databases
    SWISS-2DPAGE Get region on 2D PAGE
    Organism-specific gene databases
    EchoBASE EB1412;-.
    EcoGene EG11442;gcvT.
    HOGENOM [Family / Alignment / Tree]
    Family and domain databases
    HAMAP MF_00259-;1.
    PBIL [Family/Alignment/Tree]
    InterPro IPR006222;GCV_T.
    IPR006223;GcvT.
    Graphical view of domain structure
    Pfam PF01571;GCV_T;1.
    Pfam graphical view of domain structure
    TIGRFAMs TIGR00528;gcvT;1.
    BLOCKS P27248
    Genome annotation databases
    GenomeReviews U00096_GR;b2905.
    Other
    ProtoNet P27248
    UniRef View cluster of proteins with at least 50% / 90% / 100% identity
    Keyword
    3D-structure; Aminotransferase; Complete proteome; Direct protein sequencing; Transferase;
    Features
    Feature table viewer Feature aligner
    Key  From   To Length  Description  FTId
    INIT_MET    0     0      
    CHAIN    1   363    363  Aminomethyltransferase  PRO_0000122554
    TURN    5     6      2    
    HELIX    7    12      6    
    TURN   13    14      2    
    STRAND   16    20      5    
    TURN   21    22      2    
    STRAND   23    30      8    
    HELIX   32    41     10    
    STRAND   42    42      1    
    STRAND   44    47      4    
    TURN   49    50      2    
    STRAND   51    58      8    
    TURN   59    60      2    
    HELIX   61    68      8    
    STRAND   69    71      3    
    HELIX   73    75      3    
    STRAND   78    78      1    
    TURN   79    80      2    
    STRAND   81    88      8    
    TURN   90    91      2    
    STRAND   92    92      1    
    STRAND   94   104     11    
    TURN  105   106      2    
    STRAND  107   112      6    
    HELIX  114   131     18    
    TURN  132   132      1    
    STRAND  134   137      4    
    TURN  139   140      2    
    STRAND  141   148      8    
    TURN  149   150      2    
    HELIX  151   156      6    
    TURN  157   158      2    
    HELIX  161   167      7    
    TURN  168   169      2    
    STRAND  172   178      7    
    TURN  179   180      2    
    STRAND  181   184      4    
    STRAND  188   191      4    
    STRAND  193   199      7    
    HELIX  200   212     13    
    TURN  213   214      2    
    STRAND  216   217      2    
    HELIX  220   229     10    
    TURN  230   231      2    
    TURN  235   237      3    
    STRAND  238   238      1    
    TURN  241   242      2    
    STRAND  243   243      1    
    HELIX  245   247      3    
    TURN  248   249      2    
    HELIX  251   253      3    
    STRAND  258   258      1    
    TURN  259   260      2    
    TURN  264   265      2    
    HELIX  266   275     10    
    STRAND  279   285      7    
    STRAND  287   289      3    
    TURN  293   294      2    
    STRAND  296   300      5    
    TURN  302   303      2    
    STRAND  306   317     12    
    TURN  318   321      4    
    STRAND  322   329      8    
    TURN  331   332      2    
    STRAND  335   341      7    
    TURN  342   343      2    
    STRAND  344   351      8    
    STRAND  353   353      1    
    STRAND  356   357      2    
    TURN  358   359      2    
    STRAND  360   361      2    
    Sequence information
    Length: 363AA [This is the length of the unprocessed precursor] Molecular weight: 40016Da [This is the MW of the unprocessed precursor] CRC64: 708CCA55FA1F7F20[This is a checksum on the sequence]
            10         20         30         40         50         60
    AQQTPLYEQH TLCGARMVDF HGWMMPLHYG SQIDEHHAVR TDAGMFDVSH MTIVDLRGSR
            70         80         90        100        110        120
    TREFLRYLLA NDVAKLTKSG KALYSGMLNA SGGVIDDLIV YYFTEDFFRL VVNSATREKD
           130        140        150        160        170        180
    LSWITQHAEP FGIEITVRDD LSMIAVQGPN AQAKAATLFN DAQRQAVEGM KPFFGVQAGD
           190        200        210        220        230        240
    LFIATTGYTG EAGYEIALPN EKAADFWRAL VEAGVKPCGL GARDTLRLEA GMNLYGQEMD
           250        260        270        280        290        300
    ETISPLAANM GWTIAWEPAD RDFIGREALE VQREHGTEKL VGLVMTEKGV LRNELPVRFT
           310        320        330        340        350        360
    DAQGNQHEGI ITSGTFSPTL GYSIALARVP EGIGETAIVQ IRNREMPVKV TKPVFVRNGK
    
    AVA
    P27248 in FASTA format