Entry Information
Protein Name Glf
Accession No P37747
Protein description UDP-galactopyranose mutase
pI/Mw (Da)
Theoretical Experimental
6.62/42965.88 -
Protein function and expression Involved in the conversion of UDP-galactose proton symporter (GalP) into UDP-GalF through a 2-keto intermediate for lipopolysaccharide biosynthesis.
Reference(s) Lopez-Campistrous, A., P. Semchuk, L. Burke, T. Palmer-Stone, S. J. Brokx, G. Broderick, D. Bottorff, S. Bolch, J. H. Weiner, and M. J. Ellison. 2005. Localization, annotation & comparison of the Escherichia coli K-12 proteome under two states of growth. Mol. Cell. Proteomics 4:1205-1209. (NCBI)

Swiss-Prot Database Reference

Entry information
Entry Name GLF_ECOLI
Primary accession number P37747
Secondary accession numbers
Integrated into Swiss-Prot on 1994-10-01
Sequence was last modified on 1994-10-01 (Sequence version 1)
Annotations were last modified on 2006-09-05 (Entry version 60)
Name and origin of the protein
Protein name UDP-galactopyranose mutase
Synonyms EC 5.4.99.9 UDP-galactopyranose mutase
Gene name Name : glf
OrderedLocusName : b2036
From Escherichia coli [TaxID:562] [HAMAP proteome]
Taxonomy Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia;
References
[1] NUCLEOTIDE SEQUENCE [GENOMIC DNA].
strain = K12 / W3110 / ATCC 27325 / DSM 5911
PubMed=7517390[NCBI, ExPASy, EBI, Israel, Japan]
Yao Z. , Valvano M.A. ,
"Genetic analysis of the O-specific lipopolysaccharide biosynthesis region (rfb) of Escherichia coli K-12 W3110: identification of genes that confer group 6 specificity to Shigella flexneri serotypes Y and 4a."
J. Bacteriol.176:4133-4143(1994) 
[2] NUCLEOTIDE SEQUENCE [GENOMIC DNA].
strain = K12 / WG1
PubMed=7517391[NCBI, ExPASy, EBI, Israel, Japan]
Stevenson G. , Neal B. , Liu D. , Hobbs M. , Packer N.H. , Batley M. , Redmond J.W. , Lindquist L. , Reeves P.R. ,
"Structure of the O antigen of Escherichia coli K-12 and the sequence of its rfb gene cluster."
J. Bacteriol.176:4144-4156(1994) 
[3] NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
strain = K12 / W3110 / ATCC 27325 / DSM 5911
DOI=10.1093/dnares/3.6.379;PubMed=9097040[NCBI, ExPASy, EBI, Israel, Japan]
Itoh T. , Aiba H. , Baba T. , Fujita K. , Hayashi K. , Inada T. , Isono K. , Kasai H. , Kimura S. , Kitakawa M. , Kitagawa M. , Makino K. , Miki T. , Mizobuchi K. , Mori H. , Mori T. , Motomura K. , Nakade S. , Nakamura Y. , Nashimoto H. , Nishio Y. , Oshima T. , Saito N. , Sampei G. , Seki Y. , Sivasundaram S. , Tagami H. , Takeda J. , Takemoto K. , Wada C. , Yamamoto Y. , Horiuchi T. ,
"A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map."
DNA Res.3:379-392(1996) 
[4] NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
strain = K12 / MG1655 / ATCC 47076
DOI=10.1126/science.277.5331.1453;PubMed=9278503[NCBI, ExPASy, EBI, Israel, Japan]
Blattner F.R. , Plunkett G. III , Bloch C.A. , Perna N.T. , Burland V. , Riley M. , Collado-Vides J. , Glasner J.D. , Rode C.K. , Mayhew G.F. , Gregor J. , Davis N.W. , Kirkpatrick H.A. , Goeden M.A. , Rose D.J. , Mau B. , Shao Y. ,
"The complete genome sequence of Escherichia coli K-12."
Science277:1453-1474(1997) 
[5] NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
strain = K12 / W3110 / ATCC 27325 / DSM 5911
DOI=10.1038/msb4100049;
Hayashi K. , Morooka N. , Yamamoto Y. , Fujita K. , Isono K. , Choi S. , Ohtsubo E. , Baba T. , Wanner B.L. , Mori H. , Horiuchi T. ,
"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Mol. Syst. Biol.2:E1-E5(2006) 
[6] PROTEIN SEQUENCE OF 1-15, CHARACTERIZATION, AND MASS SPECTROMETRY.PubMed=8576037[NCBI, ExPASy, EBI, Israel, Japan]
Nassau P.M. , Martin S.L. , Brown R.E. , Weston A. , Monsey D. , McNeil M.R. , Duncan K. ,
"Galactofuranose biosynthesis in Escherichia coli K-12: identification and cloning of UDP-galactopyranose mutase."
J. Bacteriol.178:1047-1052(1996) 
Comments
  • FUNCTION : Involved in the conversion of UDP-GalP into UDP-GalF through a 2-keto intermediate
  • CATALYTIC ACTIVITY : UDP-D-galactopyranose = UDP-D-galacto-1,4-furanose
  • COFACTOR : FAD
  • PATHWAY : Lipopolysaccharide biosynthesis
  • INTERACTION:
        P11868:tdcD; NbExp=1; InAct=EBI-558730, EBI-553884
  • MASS SPECTROMETRY : MW=42960.0; MW_ERR=8.0; METHOD=Electrospray; RANGE=1-367;NOTE=Ref.6
  • Copyright
    Copyrighted by the UniProt Consortium, see http://www.uniprot.org/. Distributed under the Creative Commons Attribution-NoDerivs License.
    Cross-reference
    Sequence databases
    EMBL U03041;AAC31632.1;-;Genomic_DNA [EMBL/GenBank/DDBJ][CoDingSequence].
    U09876;AAB88403.1;-;Genomic_DNA [EMBL/GenBank/DDBJ][CoDingSequence].
    U00096;AAC75097.1;-;Genomic_DNA [EMBL/GenBank/DDBJ][CoDingSequence].
    AP009048;BAA15878.1;-;Genomic_DNA [EMBL/GenBank/DDBJ][CoDingSequence].
    PIR I69653;I69653.
    3D structure databases
    PDB 1I8T;X-ray;A/B=1-367[ExPASy/RCSB/EBI].
    Detailed list of linked structures
    ModBase P37747
    Protein-protein interaction databases
    IntAct P37747;-.
    DIP P37747
    Enzyme and pathway databases
    BioCyc EcoCyc:GALPMUT-MONOMER;-.
    2D gel databases
    SWISS-2DPAGE Get region on 2D PAGE
    Organism-specific gene databases
    EchoBASE EB1924;-.
    EcoGene EG11981;glf.
    HOGENOM [Family / Alignment / Tree]
    Family and domain databases
    InterPro IPR013027;FAD_pyr_redox.
    IPR004379;UDP-GALP_mutase.
    Graphical view of domain structure
    Pfam PF03275;GLF;1.
    PF07992;Pyr_redox_2;1.
    Pfam graphical view of domain structure
    TIGRFAMs TIGR00031;UDP-GALP_mutase;1.
    BLOCKS P37747
    Genome annotation databases
    GenomeReviews U00096_GR;b2036.
    Ontology
    GO GO:0005515;Molecular Function:protein binding(inferred from physical interaction)
    QuickGo View.
    Other
    ProtoNet P37747
    UniRef View cluster of proteins with at least 50% / 90% / 100% identity
    Keyword
    3D-structure; Complete proteome; Direct protein sequencing; FAD; Flavoprotein; Isomerase; Lipopolysaccharide biosynthesis;
    Features
    Feature table viewer Feature aligner
    Key  From   To Length  Description  FTId
    CHAIN    1   367    367  UDP-galactopyranose mutase  PRO_0000087508
    NP_BIND    3    31     29  FAD(potential)  
    STRAND    2     7      6    
    STRAND   10    10      1    
    HELIX   11    23     13    
    TURN   24    24      1    
    STRAND   27    30      4    
    STRAND   32    37      6    
    HELIX   38    40      3    
    STRAND   42    45      4    
    TURN   46    47      2    
    STRAND   48    51      4    
    TURN   52    53      2    
    STRAND   54    54      1    
    STRAND   58    61      4    
    HELIX   63    70      8    
    TURN   71    72      2    
    STRAND   73    73      1    
    STRAND   75    75      1    
    STRAND   83    86      4    
    TURN   87    88      2    
    STRAND   89    96      8    
    HELIX   97   104      8    
    HELIX  109   119     11    
    TURN  120   123      4    
    STRAND  130   130      1    
    HELIX  131   147     17    
    TURN  148   148      1    
    HELIX  149   156      8    
    STRAND  157   158      2    
    HELIX  160   162      3    
    STRAND  163   163      1    
    TURN  165   166      2    
    STRAND  167   168      2    
    STRAND  173   173      1    
    STRAND  175   177      3    
    STRAND  181   181      1    
    STRAND  185   188      4    
    STRAND  190   190      1    
    TURN  191   192      2    
    HELIX  194   202      9    
    TURN  203   204      2    
    STRAND  205   208      4    
    STRAND  210   210      1    
    HELIX  213   215      3    
    STRAND  216   216      1    
    HELIX  217   221      5    
    TURN  222   223      2    
    STRAND  224   229      6    
    STRAND  231   231      1    
    HELIX  233   236      4    
    TURN  237   241      5    
    STRAND  246   259     14    
    STRAND  261   269      9    
    TURN  272   273      2    
    STRAND  274   275      2    
    STRAND  277   281      5    
    HELIX  282   285      4    
    STRAND  286   286      1    
    STRAND  291   291      1    
    STRAND  293   302     10    
    TURN  305   306      2    
    STRAND  307   307      1    
    STRAND  311   312      2    
    STRAND  315   315      1    
    HELIX  316   331     16    
    TURN  333   334      2    
    STRAND  335   337      3    
    STRAND  339   339      1    
    TURN  340   344      5    
    STRAND  345   345      1    
    HELIX  349   364     16    
    STRAND  365   365      1    
    Sequence information
    Length: 367AA [This is the length of the unprocessed precursor] Molecular weight: 42966Da [This is the MW of the unprocessed precursor] CRC64: C62F67A4682B2A06[This is a checksum on the sequence]
            10         20         30         40         50         60
    MYDYIIVGSG LFGAVCANEL KKLNKKVLVI EKRNHIGGNA YTEDCEGIQI HKYGAHIFHT
            70         80         90        100        110        120
    NDKYIWDYVN DLVEFNRFTN SPLAIYKDKL FNLPFNMNTF HQMWGVKDPQ EAQNIINAQK
           130        140        150        160        170        180
    KKYGDKVPEN LEEQAISLVG EDLYQALIKG YTEKQWGRSA KELPAFIIKR IPVRFTFDNN
           190        200        210        220        230        240
    YFSDRYQGIP VGGYTKLIEK MLEGVDVKLG IDFLKDKDSL ASKAHRIIYT GPIDQYFDYR
           250        260        270        280        290        300
    FGALEYRSLK FETERHEFPN FQGNAVINFT DANVPYTRII EHKHFDYVET KHTVVTKEYP
           310        320        330        340        350        360
    LEWKVGDEPY YPVNDNKNME LFKKYRELAS REDKVIFGGR LAEYKYYDMH QVISAALYQV
    
    KNIMSTD
    P37747 in FASTA format