Entry Information
Protein Name GlgS
Accession No P26649
Protein description Glycogen synthesis protein GlgS
pI/Mw (Da)
Theoretical Experimental
5.38/7891.88 -
Protein function and expression Involved in glycogen synthesis. Induced strongly at the early stationary phase and also less osmotic induction. Regulated by σS and cAMP-CRP.
Reference(s) Hengge-Aronis, R. 1996. Regulation of gene expression during entry into stationary phase, p. 1497-1512. In F. C. Neidhardt, R. Curtiss III, J. L. Ingraham, E. C. C. Lin, K. B. Low, B. Magasanik, W. S. Reznikoff, M. Riley, M. Schaechter, and H. E. Umbarger (ed.), Escherichia coli and Salmonella typhimurium: cellular and molecular biology, 2nd ed., vol. 1. ASM Press, Washington, D.C. (NCBI)

Hengge-Aronis, R., R. Lange, N. Henneberg, and D. Fischer. 1993. Osmotic regulation of rpoS-dependent genes in Escherichia coli. J. Bacteriol. 175:259-265. (NCBI)

Swiss-Prot Database Reference

Entry information
Entry Name GLGS_ECOLI
Primary accession number P26649
Secondary accession numbers
Integrated into Swiss-Prot on 1992-08-01
Sequence was last modified on 1992-08-01 (Sequence version 1)
Annotations were last modified on 2006-09-05 (Entry version 43)
Name and origin of the protein
Protein name Glycogen synthesis protein glgS
Synonyms Glycogen synthesis protein glgS
Gene name Name : glgS
OrderedLocusName : b3049
From Escherichia coli [TaxID:562] [HAMAP proteome]
Taxonomy Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia;
References
[1] NUCLEOTIDE SEQUENCE [GENOMIC DNA].
strain = K12
PubMed=1324388[NCBI, ExPASy, EBI, Israel, Japan]
Hengge-Aronis R. , Fischer D. ,
"Identification and molecular analysis of glgS, a novel growth-phase-regulated and rpoS-dependent gene involved in glycogen synthesis in Escherichia coli."
Mol. Microbiol.6:1877-1888(1992) 
[2] NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
strain = K12 / W3110 / ATCC 27325 / DSM 5911
DOI=10.1093/dnares/4.2.91;PubMed=9205837[NCBI, ExPASy, EBI, Israel, Japan]
Yamamoto Y. , Aiba H. , Baba T. , Hayashi K. , Inada T. , Isono K. , Itoh T. , Kimura S. , Kitagawa M. , Makino K. , Miki T. , Mitsuhashi N. , Mizobuchi K. , Mori H. , Nakade S. , Nakamura Y. , Nashimoto H. , Oshima T. , Oyama S. , Saito N. , Sampei G. , Satoh Y. , Sivasundaram S. , Tagami H. , Takahashi H. , Takeda J. , Takemoto K. , Uehara K. , Wada C. , Yamagata S. , Horiuchi T. ,
"Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features."
DNA Res.4:91-113(1997) 
[3] NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
strain = K12 / MG1655 / ATCC 47076
DOI=10.1126/science.277.5331.1453;PubMed=9278503[NCBI, ExPASy, EBI, Israel, Japan]
Blattner F.R. , Plunkett G. III , Bloch C.A. , Perna N.T. , Burland V. , Riley M. , Collado-Vides J. , Glasner J.D. , Rode C.K. , Mayhew G.F. , Gregor J. , Davis N.W. , Kirkpatrick H.A. , Goeden M.A. , Rose D.J. , Mau B. , Shao Y. ,
"The complete genome sequence of Escherichia coli K-12."
Science277:1453-1474(1997) 
[4] NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
strain = K12 / W3110 / ATCC 27325 / DSM 5911
DOI=10.1038/msb4100049;
Hayashi K. , Morooka N. , Yamamoto Y. , Fujita K. , Isono K. , Choi S. , Ohtsubo E. , Baba T. , Wanner B.L. , Mori H. , Horiuchi T. ,
"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Mol. Syst. Biol.2:E1-E5(2006) 
[5] STRUCTURE BY NMR.PubMed=9208918[NCBI, ExPASy, EBI, Israel, Japan]
Beglova N. , Fischer D. , Hengge-Aronis R. , Gehring K. ,
"1H, 15N and 13C NMR assignments, secondary structure and overall topology of the Escherichia coli GlgS protein."
Eur. J. Biochem.246:301-310(1997) 
Comments
  • FUNCTION : Involved in glycogen synthesis. May be involved in glycogen priming
  • SIMILARITY : Belongs to the glgS family
  • Copyright
    Copyrighted by the UniProt Consortium, see http://www.uniprot.org/. Distributed under the Creative Commons Attribution-NoDerivs License.
    Cross-reference
    Sequence databases
    EMBL Z11885;CAA77940.1;-;Genomic_DNA [EMBL/GenBank/DDBJ][CoDingSequence].
    U00096;AAC76085.1;-;Genomic_DNA [EMBL/GenBank/DDBJ][CoDingSequence].
    AP009048;BAA16577.1;-;Genomic_DNA [EMBL/GenBank/DDBJ][CoDingSequence].
    PIR S25201;S25201.
    3D structure databases
    PDB 1RRZ;NMR;A=1-66[ExPASy/RCSB/EBI].
    Detailed list of linked structures
    ModBase P26649
    Protein-protein interaction databases
    DIP P26649
    Enzyme and pathway databases
    BioCyc EcoCyc:EG11381-MONOMER;-.
    2D gel databases
    SWISS-2DPAGE Get region on 2D PAGE
    Organism-specific gene databases
    EchoBASE EB1355;-.
    EcoGene EG11381;glgS.
    HOGENOM [Family / Alignment / Tree]
    Family and domain databases
    HAMAP MF_00525-;1.
    PBIL [Family/Alignment/Tree]
    BLOCKS P26649
    Genome annotation databases
    GenomeReviews U00096_GR;b3049.
    Other
    ProtoNet P26649
    UniRef View cluster of proteins with at least 50% / 90% / 100% identity
    Keyword
    3D-structure; Complete proteome; Glycogen biosynthesis;
    Features
    Feature table viewer Feature aligner
    Key  From   To Length  Description  FTId
    CHAIN    1    66     66  Glycogen synthesis protein glgS  PRO_0000071599
    TURN    3     6      4    
    HELIX    7    24     18    
    TURN   25    25      1    
    HELIX   30    36      7    
    TURN   39    40      2    
    HELIX   41    59     19    
    STRAND   61    62      2    
    STRAND   64    64      1    
    Sequence information
    Length: 66AA [This is the length of the unprocessed precursor] Molecular weight: 7892Da [This is the MW of the unprocessed precursor] CRC64: D047AB6E7AD67523[This is a checksum on the sequence]
            10         20         30         40         50         60
    MDHSLNSLNN FDFLARSFAR MHAEGRPVDI LAVTGNMDEE HRTWFCARYA WYCQQMMQAR
    
    ELELEH
    P26649 in FASTA format